METHODOLOGICAL CHALLENGES OF DIGITAL PCR DETECTION OF THE HISTONE H3 K27M SOMATIC VARIANT IN CEREBROSPINAL FLUID

Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid

Cell-free DNA (cfDNA) in body fluids is invaluable for cancer diagnostics.Despite the impressive potential of liquid biopsies for the diagnostics of central nervous system (CNS) tumors, a number of challenges prevent introducing this approach into routine laboratory practice.In this study, we adopt a protocol for sensitive detection of the H3 K27M

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Moyamoya Disease: Epidemiology, Clinical Features, and Diagnosis

Moyamoya disease (MMD) is a chronic, occlusive cerebrovascular disease characterized by progressive stenosis at the terminal portion of the internal carotid artery and an abnormal vascular network at the base of the brain.Although its etiology remains unknown, recent genetic studies identified RNF213 in the 17q25-ter region CANDLE HOLDER as an impo

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Hydraulic Stability of the Armor Layer of Overtopped Breakwaters

Mound breakwaters with significant overtopping rates in depth-limited conditions are common in practice due to social concern about the visual impact of coastal structures and sea level rise due to climatic change.For overtopped mound breakwaters, the highest waves pass over the crest producing armor damage, not only to the front slope, but also to

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